Aquatic Toxicity Testing

The ATCL performs aquatic toxicity tests on a variety of fish species including largemouth bass, fathead minnow, sheepshead minnow, Menidia beryllina and mosquitofish. In addition several bioanalytical and molecular assays are available.

Bioanalytical Assays

Bioanalytical Assays Cost per sample/test
Vtg ELISA/ sample $30
SDS Gel $80
Western blot $100
Vtg purification $500
Antibody screen (West/ELISA) $300
EIA for E2, T, 11KT/sample $35
Histology $100
Vtg mRNA $60
Invitrogen transactivation assays/receptor type $1,000
FP-ER- binding assay $200

Toxicity Assays

Toxicity Assays COST PER SAMPLE/TEST
Exposures static (2 changes/day) 2-4 d Control and 1 effluent $2,500
additional effluents up to 5 $1500
Cumulative egg production assay, 14 days $15,000
ELS Larval toxicity assay 96 h growth, survival $5,000
ZF ELS embryo toxicity test, 96 h growth, survival, deformities, hatching $5,000
Mosquitofish gonapodium 7 day, 5 replicates $1,250

Molecular Assays

Molecular Assays COST PER SAMPLE/TEST
Microarrays, minimum 8 samples $500