John A. Bowden, Ph.D.

Dr. John Bowden, Dept. of Physiological Sciences

Assistant Professor

Center for Environmental and Human Toxicology &
Department of Physiological Sciences
1333 Center Drive, BSB
Gainesville, FL 32611
BSB Office: 352-294-4063
2187 Mowry Road, Bldg 471
CEHT Office: 352-294-4064

Joint Appointment
Department of Chemistry
Analytical Division



  • Ph.D. in Analytical Chemistry, University of Florida, 2008
  • B.S. in Chemistry, University of Florida, 2003

Honors and Awards

  • Mentor Accolade – Material Measurement Laboratory (NIST), 2017
  • Measurement Science Excellence Accolade – Material Measurement Laboratory (NIST), 2016
  • NIST/NRC Postdoctoral Research Fellowship/Associateship, 2011
  • HHMI Science for Life Graduate Mentor Award, 2009
  • Chemistry Department Teaching Award, University of Florida, 2007
  • Graduate Student Teaching Assistant Award, College of Liberal Arts and Sciences, University of Florida, 2006
  • Chemistry Department Teaching Award, University of Florida, 2004

Professional Experience

  • July 2013 – July 2018 Research Chemist, Marine Biochemical Sciences Group, Chemical Sciences Division, National Institute of Standards and Technology, Charleston, SC
  • July 2011 – July 2013 NIST/NRC Postdoctoral Fellow, Environmental Chemical Sciences Group, Chemical Sciences Division, National Institute of Standards and Technology, Charleston, SC
  • June 2009 – June 2011 Postdoctoral Fellow, Department of Biochemistry and Molecular Biology, Saint Louis University, St. Louis, MO

Research Interests

The research I am pursuing in my laboratory focuses on employing mass spectrometric methods at the chem/bio interface, with a long-standing interest in endocrine disruption and environmental chemistry. A new drive in the laboratory is focused on studying the interplay between external measures of exposure (e.g., anthropogenic contaminants) and internal measures of exposure (e.g., lipids, hormones), in the context of health and disease. The tools we use to study these relationships are either gas or liquid chromatography and mass spectrometry (employing both targeted and non-targeted workflows). Beyond the implementation of these workflows, a considerable effort in the laboratory also focuses on improving key metrological aspects, including method development and optimization, quantitation, and quality control. Current work primarily employs workflows capable of performing lipidomics, metabolomics, and the measurement of chemicals of emerging concern (e.g., perfluorinated chemicals). Application areas are varied, encompassing environmental sampling, nutritional studies, lab-based models, and both wildlife and human health.

Selected Publications

  • Candice Z. Ulmer, Christina M. Jones, Richard A. Yost, Timothy J. Garrett, John A. Bowden. Optimization of Folch, Bligh-Dyer, and Matyash Lipid Extraction Solvent to Sample Ratios for Human Plasma-Based Lipidomics Studies. Analytica Chimica Acta – Invited – Special Issue on Analytical Metabolomics (just accepted)
  • Jacqueline T. Bangma, Jessica L. Reiner, Russell H. Lowers, Theresa M. Cantu, Jacob Scott, Jeffrey Korte, Doug M. Scheidt, Chris McDonough, Jonathan Tucker, Eric Reyier, Bonnie Ahr, Brenton Back, Douglas H. Adams, and John A. Bowden. Perfluorinated Alkyl Acids and Fecundity Assessment in Striped mullet (Mugil cephalus) at Merritt Island National Wildlife Refuge. Science of the Total Environment 2018 Apr 1;619-620:740-747. doi: 10.1016/j.scitotenv.2017
  • John A. Bowden, Alan Heckert, Candice Z. Ulmer, Christina M. Jones, Jeremy P. Koelmel, et al. Harmonizing Lipidomics: NIST Interlaboratory Comparison Exercise for Lipidomics using Standard Reference Material 1950 – Metabolites in Frozen Human Plasma. Journal of Lipid Research, 2017 Dec;58(12):2275-2288. doi: 10.1194/jlr.M079012
  • Jeremy P. Koelmel, Nicholas M. Kroeger, Candice Z. Ulmer, John A. Bowden, Rainey E. Patterson, Jason A. Cochran, Christopher W. W. Beecher, Timothy J. Garrett, Richard A. Yost. LipidMatch: an automated workflow for rule-based lipid identification using untargeted high-resolution tandem mass spectrometry data. BMC Bioinformatics (2017) 18:331. DOI: 10.1186/s12859-017-1744-3
  • Jacqueline T. Bangma, Jessica L. Reiner, Hannes Botha, Theresa M. Cantu, Marco A. Gouws, Matthew P. Guillette, Jeremy P. Koelmel, Wilmien J. Luus-Powell, Jan Myburgh, Olivia Rynders, Joseph R. Sara, Willem J. Smit, John A. Bowden. Tissue distribution of perfluoroalkyl acids and health status in wild Mozambique tilapia (Oreochromis mossambicus) from Loskop Dam, Mpumalanga, South Africa. Journal of Environmental Sciences 61:59-67. DOI:10.1016/j.jes.2017.03.041
  • Jacqueline T. Bangma, Jessica L. Reiner, Martin Jones, Russell H. Lowers, Frances Nilsen, Thomas R. Rainwater, Stephen Somerville, Louis J. Guillette, John A. Bowden: Variation in perfluoroalkyl acids in the American alligator (Alligator mississippiensis) at Merritt Island National Wildlife Refuge. Chemosphere 01/2017; 166:72-79.

Additional publications here