The aquatic toxicology laboratory performs aquatic toxicity tests on a variety of fish species including largemouth bass, fathead minnow, sheepshead minnow, Menidia beryllina and mosquitofish. In addition several bioanalytical and molecular assays are available.
Bioanalytical Assays
| Bioanalytical Assays | TOTAL PER SAMPLE/TEST |
|---|---|
| Vtg ELISA/ sample | $30.00 |
| SDS Gel | $80.00 |
| Western blot | $100.00 |
| Vtg purification | $500.00 |
| Antibody screen (West/ELISA) | $300.00 |
| Vtg mRNA by QPCR, minimum 4 samples | $60.00 per sample |
| Invitrogen transactivation assays/receptor type | $1,000.00 |
| FP-ER-binding assay | $200.00 |
Toxicity Assays
| Toxicity Assays | TOTAL PER SAMPLE/TEST |
|---|---|
| Exposures static (2 changes/day) 2-4 d Control and 1 effluent | $2,500.00 |
| – Additional effluents up to 5 | $1,500.00 |
| Cumulative egg production assay 14 days | $15,000.00 |
| ELS Larval toxicity assay 96 h growth, survival | $3,000.00 |
| ZF ELS embryo toxicity test 96 h growth, survival, deformities, hatching | $3,000.00 |
| Mosquitofish gonapodium 7 day 5 replicates | $2,000.00 |
Molecular Assays
| Molecular Assays | TOTAL PER SAMPLE/TEST |
|---|---|
| qPCR for specific genes of interest – minimum 4 samples | $60 per sample |
| RNAseq – quadruplicate replicates | $10,000 |